| Range |
~150,000 ribosomes/cell
|
| Organism |
Fission yeast Schizosaccharomyces pombe |
| Reference |
Marguerat S et al., Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells. Cell. 2012 Oct 26 151(3):671-83. doi: 10.1016/j.cell.2012.09.019. p.677 right column 2nd paragraphPubMed ID23101633
|
| Method |
p.677 right column 2nd paragraph:"[Investigators] compared protein concentrations with cellular ‘‘landmarks’’ for meaningful biological context (Figure 5E). The ribosome is
a large complex composed of single copies of multiple proteins and rRNAs. Thus, transcriptome and proteome data correctly calibrated relative to each other should arrive at similar estimates
for total ribosome numbers, allowing cross-validation of [their] two independent data sets." |
| Comments |
p.677 right column 2nd paragraph:"Reassuringly, the numbers for most ribosomal proteins were consistent with the numbers for different rRNAs (Figure 5E), indicating that there are 1–2×10^5 ribosomes in an average proliferating cell. This number is comparable to an electron microscopy estimate (~5×10^5 ribosomes/cell Maclean, 1965 BNID 108292). For further confirmation, [investigators] calculated the total number of ribosomes associated with mRNAs by multiplying copy numbers of all individual mRNAs with their associated ribosome numbers obtained from polysome profiling (Lackner et al., 2007), resulting in a total of ~1.5×10^5 ribosomes/cell." |
| Entered by |
Uri M |
| ID |
114958 |