Number of ribosomes

Range rich medium 83,200±7,400: poor medium 25,600±5,000 ribosomes/cell
Organism Bacteria Staphylococcus aureus
Reference Martin SE, Iandolo JJ. Translational Control of Protein Synthesis in Staphylococcus aureus. J Bacteriol. 1975 Jun122(3):1136-43 p.1138 right column bottom paragraph and p.1140 Table 2PubMed ID1150618
Method P.1138 right column bottom paragraph: "To estimate the in vivo step times (ref 20), a determination of the amounts of RNA and protein per cell was performed (Table 1). Cells grown in rich medium contained more abundant amounts of all macromolecules. From these data, and the observation that, respectively, 76% and 62% of the total RNA was present as ribosomal RNA in rich and poor medium-derived cells, an estimation of the number of ribosomes per cell was possible (Table 1)."
Comments P.1138 right column bottom paragraph: "Cells grown in rich medium contained approximately 83,200 ribosomes/cell, whereas poor medium-derived cells had only 25,600 ribosomes/cell."
Entered by Uri M
ID 114938