Characteristic of T. delicata Aio [arsenite oxidase] compared with Aio from other mesophilic organisms

Range Table - link
Organism bacteria
Reference Teoh WK et al., Characterization of Thiomonas delicata arsenite oxidase expressed in Escherichia coli. 3 Biotech. 2017 Jun7(2):97. doi: 10.1007/s13205-017-0740-7 p.5 table 1PubMed ID28560637
Primary Source See refs beneath table
Method Abstract: "In the present study, the arsenite oxidase gene (aioBA) was cloned from Thiomonas delicata DSM 16361, expressed heterologously in E. coli and purified to homogeneity."
Comments P.4 left column bottom paragraph: "By utilizing DCPIP [artificial electron acceptor 2,6-dichlorophenolindophenol] as the artificial electron acceptor, the recombinant Aio [arsenite oxidase] showed typical Michaelis–Menten kinetics with substrate arsenite, with Vmax of 4 ± 0.07 µmol arsenite oxidized/min/mg of proteins and Km 14.2 ± 1.03 µM (Fig. 2). Km of the recombinant Aio was found to be higher than Aio from A. faecalis, but lower than Arthrobacter sp. 15b and NT-26 (Table 1). High Km indicated the lower affinity of the arsenite oxidase towards arsenite substrate. As for the Vmax, the T. delicata Aio displayed a higher specific activity than the other arsenite oxidases." See note beneath table
Entered by Uri M
ID 114119