Growth phenotypes and doubling times of strains

Range Table - link
Organism Bacteria Rhodospirillum spp.
Reference Dey S et al., In Vivo Studies in Rhodospirillum rubrum Indicate That Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) Catalyzes Two Obligatorily Required and Physiologically Significant Reactions for Distinct Carbon and Sulfur Metabolic Pathways. J Biol Chem. 2015 Dec 25 290(52):30658-68. doi: 10.1074/jbc.M115.691295. p.30663 table 1PubMed ID26511314
Primary Source See refs beneath table
Method P.30659 left column 3rd paragraph: "In the current study, [investigators] show through in vivo experiments that representatives of all bona fide Rubisco [ribulose-1,5-bisphosphate carboxylase/oxygenase] forms (I, II, and III) catalyze key reactions for both CO2 fixation and MTA [5-methylthioadenosine] metabolism. Complete inactivation of the CO2 fixation reaction does not prevent or affect Rubisco-dependent MTA metabolism. In addition, knock-out metabolomics revealed that Rubisco's role in anaerobic MTA metabolism is potentially linked to S-methyl-cysteine and S-methyl-mercaptolactate production. These results suggest that the quintessential carbon fixation enzyme, Rubisco, also catalyzes an essential reaction required for anaerobic MTA metabolism."
Comments P.30660 left column top paragraph: "Bacterial growth was monitored at OD660 nm, and bacterial growth curves were fit by non-linear weighted regression (MATLAB, MathWorks) to a sigmoidal-logistic model (ref 25) to measure the growth rate (Table 1)." P.30660 right column 2nd paragraph: "To further address the requirement of Rubisco for anaerobic MTA metabolism, [investigators] determined the ability of the Rubisco deletion strain, I19NifA (ΔcbbM, nifA-M173V), to grow anaerobically on MTA as sole sulfur source as compared with wild type (Fig. 2C). Rubisco knock-out strains of R. rubrum are incapable of photoautotrophic growth (primary source 17, ref 28) and grow poorly under photoheterotrophic conditions using substrates such as malate as electron donor and carbon source and sulfate as the sulfur source (Fig. 2, Table 1). With such strains, the role of Rubisco and the CBB [Calvin-Benson-Bassham] pathway in balancing the redox potential of the cell is taken over by other physiological processes, such as nitrogenase-dependent hydrogen evolution (refs 28, 29)." See notes above table
Entered by Uri M
ID 112612