| Range |
Table - link
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Valm AM et al., Applying systems-level spectral imaging and analysis to reveal the organelle interactome. Nature. 2017 Jun 1 546(7656):162-167. doi: 10.1038/nature22369 Extended Data Table 1PubMed ID28538724
|
| Method |
Abstract: "Here [investigators] present a systems-level analysis of the organelle interactome using a multispectral image acquisition method that overcomes the challenge of spectral overlap in the fluorescent protein palette. [They] used confocal and lattice light sheet instrumentation and an imaging informatics pipeline of five steps to achieve mapping of organelle numbers, volumes, speeds, positions and dynamic inter-organelle contacts in live cells from a monkey fibroblast cell line." P.163 right column bottom paragraph: "To acquire three-dimensional images of organelles in live cells with high spatial and temporal resolution, [investigators] next developed a lattice light sheet (LLS) implementation of multispectral imaging using an excitation-based linear unmixing approach (Extended Data Fig. 7a–c). This resulted in 3D images (Fig. 3a, Extended Data Fig. 7d, e) and 4D videos (Supplementary Video 4) in which six organelles were distinguished." |
| Comments |
P.163 right column bottom paragraph: "The mean number, mean volume, total volume per cell of the organelles, and total cell volume in these images, as well as the speed of globular organelles from confocal images, are reported in Extended Data Table 1. These measurements revealed that the ER occupies approximately 37 times the volume of the Golgi and 9 times the volume of the mitochondria. The numbers of LDs [lipid droplets], peroxisomes and lysosomes each ranged from around 90 to 190 per cell, and the maximum speed of movement of lysosomes was twice that of LDs and peroxisomes." See notes beneath table |
| Entered by |
Uri M |
| ID |
113930 |