| Comments |
P.6 left column 3rd paragraph: "Schizosaccharomyces pombe (fission yeast) is a well-studied model organism with similar molecular pathway makeup and genetic mechanisms as those in humans [refs 89, 90]. However, little effort has been made to develop S. pombe into a suitable framework for high-value compound production [ref 91], and so efforts to increase CoQ10 in S. pombe have thus far been limited. In one study, genes encoding enzymes directly involved in CoQ10 biosynthesis (dps1 +–dlp1 +, ppt1 +, and coq3 +–coq9 +) and HMGR [HMG-CoA (3-hydroxy-3-methylglutaryl-coenzyme A) reductase][ref 83] were overexpressed. However, only overexpression of HMGR—and not the CoQ10 biosynthesis genes—led to a prominent 2.7-fold increase in CoQ10 yield (Table 1). It was posited that the lack of effect from the biosynthetic genes was because these enzymes are not rate-limiting." |