| Range |
>3 %
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Pincus D. Size doesn't matter in the heat shock response. Curr Genet. 2017 May63(2):175-178. doi: 10.1007/s00294-016-0638-7. p.176 right column top paragraphPubMed ID27502399
|
| Primary Source |
Hahn JS, Hu Z, Thiele DJ, Iyer VR (2004) Genome-wide analysis of the biology of stress responses through heat shock transcription factor. Mol Cell Biol 24 :5249–5256 DOI: 10.1128/MCB.24.12.5249-5256.2004PubMed ID15169889
|
| Comments |
P.176 right column top paragraph: "A previous report had suggested that Hsf1 regulates upward of 3 % of yeast genes (primary source), so [investigators] expected many transcripts to change upon Hsf1 nuclear depletion. Strikingly, however, only 18 genes showed both reduced transcription and total mRNA levels following rapamycin treatment under basal conditions (Fig. 1a). All of these 18 Hsf1-dependent genes (HDGs) showed strong Hsf1 binding peaks in their promoters as determined by ChIP-seq [wiki-[this] is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins], and all but one HDG encodes a chaperone or other proteostasis-related factor." |
| Entered by |
Uri M |
| ID |
113503 |