Fraction of proteome that is expressed under normal growth conditions

Value 80 %
Organism Budding yeast Saccharomyces cerevisiae
Reference Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS. Global analysis of protein expression in yeast. Nature. 2003 Oct 16 425(6959):737-41 abstractPubMed ID14562106
Method To facilitate global protein analyses, researchers have created a Saccharomyces cerevisiae fusion library where each open reading frame is tagged with a high-affinity epitope and expressed from its natural chromosomal location. Through immunodetection of the common tag, they obtain a census of proteins expressed during log-phase growth and measurements of their absolute levels.
Comments Researchers find that about 80% of the proteome is expressed during normal growth conditions
Entered by Uri M
ID 104366