Mass of protein in hepatocyte (assuming edge length of 15µm and protein concentration of 200 g/l)

Value 675 pg
Organism Unspecified
Reference Wiśniewski JR, Hein MY, Cox J, Mann M. A "proteomic ruler" for protein copy number and concentration estimation without spike-in standards. Mol Cell Proteomics. 2014 Dec13(12):3497-506. doi: 10.1074/mcp.M113.037309. p.3502 left column bottom paragraphPubMed ID25225357
Comments P.3502 left column bottom paragraph: "The total cellular protein concentration typically lies within a range of 20% to 30% (w/v) (i.e. 200 to 300 g/l, BNID 113242) in many cell types and organisms (ref 33). This constraint can be used to convert between cellular protein mass and cell volume. Hepatocytes, the predominant cell type in liver, are roughly cubical cells with a 15-μm edge length (BNID 113236). Assuming a total protein concentration of 200 g/l, this translates to 675 pg of protein per cell. This compares to [investigators'] estimate of 464 ± 35 pg total protein per average liver cell, which is reasonable given that non-hepatocytes contribute the same amount of DNA or histones but less overall protein mass."
Entered by Uri M
ID 113237