| Reference |
Capuano F, Mülleder M, Kok R, Blom HJ, Ralser M. Cytosine DNA methylation is found in Drosophila melanogaster but absent in Saccharomyces cerevisiae, Schizosaccharomyces pombe, and other yeast species. Anal Chem. 2014 Apr 15 86(8):3697-702. doi: 10.1021/ac500447w. p.3699 table 1PubMed ID24640988
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| Comments |
P.3700 left column 2nd paragraph: "Whereas, unmethylated cytidines were readily detected in all yeast species (Figure 1b, upper panel), the protocol applied on S. cerevisiae, S. boulardii, S. paradoxus, P. pastoris, and S. pombe DNA did not detect any evidence for the content of 5-methyldeoxycytidine (Figure 1b, lower panel Table 1). Taking into account the limit of detection at 250 attomol for methyldeoxycytidine, its content in yeast would be lower than 0.00002 per deoxycytidine (Table 1). Considering the importance of budding yeast in basic research and industry, the analysis was conducted on five different S. cerevisiae yeasts. These included the laboratory strains BY4741 (in its prototrophic version, BY4741-pHLUM (ref 25)), which had yielded the highest S. cerevisiae 5-meC content in the study by Tang et al. (ref 13) and the strain D273–10B that was used in the seminal study that claimed for the first time the absence of DNA methylation in S. cerevisiae.(ref 12) The measurements were conducted on DNA purified both from cells grown to stationary and exponential phase. Under both conditions DNA methylation remained undetectable. In addition, the absence of methylation was confirmed in industrial yeast. Analysis of the vine production strain AWRI 796, the lager/pilsner beer production yeast Saflager W-34/70, and the baking “dry-yeast” SCHD0308 (Ruf, Germany) revealed no evidence for DNA methylation either (Table 1)." |