Doubling times of wild-type and mutant strains grown with glucose, choline, GB [glycine betaine], or DMG [dimethylglycine] as the sole source of carbon
| Range | Table - link hours |
|---|---|
| Organism | Bacteria Pseudomonas aeruginosa |
| Reference | Wargo MJ, Szwergold BS, Hogan DA. Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism. J Bacteriol. 2008 Apr190(8):2690-9. DOI: 10.1128/JB.01393-07 p.2693 table 2PubMed ID17951379 |
| Method | P.2691 left column 4th paragraph: "Growth assays. Growth assays were conducted in 96-well polystyrene plates using a Spectromax plate reader (Molecular Devices). To obtain carbon source growth curves, cells were grown overnight in MOPS medium with 20 mM pyruvate and 5 mM glucose. The overnight cultures were pelleted, washed, and inoculated into MOPS medium with the appropriate carbon source to an initial A600 of 0.05. Plates were shaken at 37°C, and growth was measured by determining the optical density (A600) every hour. To test whether different compounds could serve as sole sources of nitrogen, growth experiments were performed in a similar manner, except that M63 medium containing 0.2% glucose but lacking ammonium chloride (ref 3) and amended with 20 mM choline, 20 mM lysine, 20 mM GB, 20 mM DMG, or 20 mM sarcosine was used. Mutants defective in PA3082 were grown on the media described above, as well as in the minimal medium described by Serra et al. (ref 33), and compared to the wild type." |
| Comments | P.2693 right column 2nd paragraph: "Consistent with the phenotypes of the transposon mutants, the P. aeruginosa ΔPA5410-PA5411 strain was defective in growth on choline or GB but could grow on DMG (Table 2). When the ΔPA5410-PA5411 strain was grown in minimal medium with glucose or pyruvate as the sole carbon source, its growth rate was indistinguishable from that of PA14 (Table 2 and data not shown). Complementation with the PA5410 and PA5411 genes on a plasmid (pGbcAB) under control of their native promoters partially restored the ability to grow on choline and GB (Table 2)." PA14 is the wildtype strain |
| Entered by | Uri M |
| ID | 112939 |