Number of random gene disruptions that are lethal out of 79 disruptions

Value 6 lethal disruptions/79 random disruptions
Organism Bacteria Bacillus subtilis
Reference Koonin EV. How many genes can make a cell: the minimal-gene-set concept. Annu Rev Genomics Hum Genet. 2000 1: 99-116. DOI: 10.1146/annurev.genom.1.1.99 p.100 3rd paragraphPubMed ID11701626
Primary Source Itaya M. 1995. An estimation of minimal genome size required for life. FEBS Lett. 362: 257–60PubMed ID7729508
Comments P.100 3rd paragraph: "A remarkable experimental study that resulted in an estimation of the minimal genome size was published at the end of the pregenomic era. Itaya has shown that of 79 random gene disruptions in Bacillus subtilis, only 6 were lethal (primary source). Furthermore, even simultaneous insertions into 33 loci have produced a viable bacterium."
Entered by Uri M
ID 112736