| Comments |
P.17 right column top paragraph: "Critical Parameters and Troubleshooting:
Consideration of EGFR expression levels—The design and interpretation of the quantitative analysis of EGFR turnover and trafficking must take into consideration the expression levels of EGFR in the cell line to be studied. Normal mammalian cells express from 5,000 (most epithelial and fibroblast cells) to 200,000 (hepatocytes) EGFR per cell. Cancer
cells express up to 2–3 × 10^6
EGFR/cell. Recombinant EGFR can be stably expressed in cells at different levels to directly analyze the dependency of the parameters of the EGFR biosynthesis, endocytosis, recycling, and degradation on the EGFR concentration in the cell." |