| Range |
Figure - link protein copies
|
| Organism |
Human Homo sapiens |
| Reference |
Itzhak DN, Tyanova S, Cox J, Borner GH. Global, quantitative and dynamic mapping of protein subcellular localization. Elife. 2016 Jun 9 5. pii: e16950. doi: 10.7554/eLife.16950. p.61 figure 5PubMed ID27278775
|
| Method |
Abstract: "[Investigators] have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. [They] initially used maps statically to generate a database with localization and absolute copy number information for over 8,700 proteins from HeLa cells, approaching comprehensive coverage. All major organelles were resolved, with exceptional prediction accuracy (estimated at >92%). Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. [They] subsequently demonstrated the dynamic capabilities of the approach by capturing translocation events following EGF [epidermal growth factor] stimulation, which [they] integrated into a quantitative model." |
| Comments |
P.15 bottom paragraph: "Dynamic organellar maps applied to EGF signaling: Here, [investigators] have used organellar maps to analyse cellular events following EGF stimulation. [They] correctly captured the endosomal transition of EGF receptor, and recruitment of signalling adaptors. Remarkably, the translocations were detected with extremely stringent FDR (false discovery rate) control, using cut-offs where [they] expect no false positives. This supports that [their] approach is capable of identifying translocation events de novo, without having to filter results based on prior knowledge. Furthermore, in combining the translocation data with protein copy number estimations, [they] provide a genuine systems-biology approach to EGF signalling at the protein level (Figure 5)." P.46 bottom paragraph: "Figure 5: Quantitative mapping of EGF-triggered subcellular translocation events. Summary of key protein translocations in HeLa cells following 20 minutes of continuous stimulation with EGF. All depicted changes were detected by organellar maps in this study they include numerous previously known as well as novel translocation events. Numbers on arrows indicate how many copies of a protein undergo the indicated movement (per cell). These estimates were also calculated from the mass spectrometry data, using the proteomic ruler approach (Wisniewski et al., 2014). Figure 4-figure supplement 3 and Supplementary files 6 (interactive database) and 7 (compact summary) show additional translocations not included here." |
| Entered by |
Uri M |
| ID |
112705 |