| Comments |
P.2926 right column bottom paragraph: "Golgi tubules had an average diameter of ∼180 nm whereas the ER network branches were ∼115 nm in diameter [BNID 112517] or a factor of RG/RE= 1.57 ± 0.2 (Fig. 4). The inverse ratio of tether forces is FE/FG = 1.63 ± 0.3 [See values in range of BNID 112516]. [Investigators] find that the two ratios (FE/FG and RG/RE) are the same within experimental error. This implies that both membrane types have approximately the same bending stiffness: BE ≈ BG ≈ 3.3 × 10^−19 N/m, similar to that of growth cone membranes and phospholipid bilayers (Dai and Sheetz, 1995a, Evans and Rawicz, 1990 Song and Waugh, 1993). Calculating the tensions explicitly using Eq. 3, [investigators] find that the tension in the ER membrane networks is TE ≈ 0.013 dyn/cm and the tension in the Golgi membrane is TG ≈ 0.005 dyn/cm. To put these tensions in perspective, membrane lysis requires a tension of 5–10 dyn/cm." |