| Comments |
P.4/21 bottom paragraph to p.6/21 top paragraph:"The S10Tnp-35 strain, where S10 location is slightly changed, displayed no μ alteration compared to the parental strain, showing that S10 precise position is not important for optimal growth and that the transposition process by itself exerts no influence on GR [growth rate] (Fig 2 and S5 Table). The GR of strain S10Tnp+166, where S10 was placed in close proximity to oriC1, did not significantly differ from the parental strain. Meanwhile, strains S10Tnp-510 and S10Tnp-1120 showed μ reductions of 7.39 ± 2.67% and 15.58 ± 3.14% respectively
(Fig 2B). Therefore, increasing distance between S10 and oriC of Chr1 (oriC1) correlated with greater μ differences. S10 transpositions to Chr2 caused significant μ reductions of 4.8 ± 1.8% and 17.29 ± 3.44% for S10TnpC2+37 and S10TnpC2+479 respectively. Hence, correlation
between distance of relocation and GR differences is also observed for this replicon (Fig 2 and S5 Table)." P.14/21 2nd paragraph:"Strains and plasmids used in this study are listed in S5 Table. Details of strain generation are in Supporting Information." Corresponding to doubling times of 38min & 147min for fast growth & slow growth, respectively. |