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The effect of ligand-binding upon the buoyant density of DNAs of different base compositions in Clostridium perfringens & Micrococcus luteus

Range	C. perfringens 1.12g/ml: M. luteus 1.13g/ml Table - link
Organism	bacteria
Reference	Ford TC, Rickwood D. Effect of ligand binding on the buoyant density of DNA in Nycodenz gradients. Nucleic Acids Res. 1984 Jan 25 12(2):1219-26. p.1221 table 1PubMed ID6320119
Comments	P.1220 bottom line to p.1221 top paragraph:"When DNA from Clostridium perfringens (70% A+T) and Micrococcus lysodeikticus (28% A+T) were banded isopycnically in Nycodenz gradients containing 10mM NaCl, both types of DNA banded at similar buoyant densities, 1.119 ± 0.001g/ml and 1.127 ± 0.001g/ml, respectively (Table 1). The density of each type of DNA and the differences between them were much less than those observed in CsCl gradients (ref 2)."
Entered by	Uri M
ID	112346