| Range |
~1 sec
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| Organism |
Unspecified |
| Reference |
Van Valen D et al., A single-molecule Hershey-Chase experiment. Curr Biol. 2012 Jul 24 22(14):1339-43. doi: 10.1016/j.cub.2012.05.023. p.1339 right column 2nd paragraphPubMed ID22727695
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| Primary Source |
[5] Yan X et al., (2005). Probing the kinetics of SYTOX Orange stain binding to double-stranded DNA with implications for DNA analysis. Anal. Chem. 77, 3554–3562.PubMed ID15924389
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| Method |
Primary source abstract:"Rapid binding kinetics of SYTOX Orange stain with double-stranded DNA (dsDNA) was revealed on the DNA fragment sizing flow cytometer." |
| Comments |
P.1339 right column 2nd paragraph:"As shown in Figure 2C, the ejection process is characterized by a loss of fluorescence intensity in the phage and a concomitant increase in fluorescence in the cellular interior (Figure 2D representative Movies S1 and S2).
The fluorescence inside the cell is diffuse: this reflects the dye molecules unbinding kinetics from the phage DNA (residence time ~1 s [primary source]) and redistributing themselves along the host genome, as verified in the Supplemental Experimental Procedures (Figures S3B and S3C)." Primary source p.3560 right column:"As shown in Figure 6a, with [investigators'] universal staining protocol of using 500 nM SYTOX Orange to stain a wide range of DNA concentrations, binding equilibrium can be reached in less than 1s." |
| Entered by |
Uri M |
| ID |
112340 |