| Range |
this investigation's measurement 2.5min: previous measurement ~8min min
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| Organism |
Bacteria Escherichia coli |
| Reference |
Chen H, Shiroguchi K, Ge H, Xie XS. Genome-wide study of mRNA degradation and transcript elongation in Escherichia coli. Mol Syst Biol. 2015 Jan 12 11(1):781. doi: 10.15252/msb.20145794. p.4 left column bottom paragraphPubMed ID25583150
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| Primary Source |
Bernstein JA, Khodursky AB, Lin P‐H, Lin‐Chao S, Cohen SN (2002) Global analysis of mRNA decay and abundance in Escherichia coli at single‐gene resolution using two‐color fluorescent DNA microarrays. Proc Natl Acad Sci USA 99: 9697–9702 & Selinger DW, Saxena RM, Cheung KJ, Church GM, Rosenow C (2003) Global RNA half‐life analysis in Escherichia coli reveals positional patterns of transcript degradation. Genome Res 13: 216–223PubMed ID12119387, 12566399
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| Method |
Abstract:"Here, [investigators] report a genome-wide RNA degradation study in Escherichia coli using RNA-seq, and present evidence that the stereotypical exponential RNA decay curve obtained using initiation inhibitor, rifampicin, consists of two phases: residual RNA synthesis, a delay in the interruption of steady state that is dependent on distance relative to the mRNA's 5' end, and the exponential decay." |
| Comments |
P.4 left column bottom paragraph:"[Investigators] average the lifetimes of the 300‐nt segments along each transcript and report these lifetimes (Fig 3A Supplementary Table S4). [Their] measurements gave an average lifetime of 2.5 min, shorter than the previous genome‐wide microarray measurements which have an average lifetime of 6 min [this is in error, should be ~8 min, see below] for genes, probably because of [their] correction for residual RNAP activity (primary sources). The distribution of lifetimes of operons and genes is similar (Supplementary Fig S4). The previously reported correlation between gene function and lifetime (primary sources) was not observed in [their] data (Supplementary Table S5)." Corrigendum to value of lifetime of 6min above is found in Chen H et al., 2015 PMID 25964259 p.1 right column 2nd paragraph:"Additionally, [investigators] would like to correct a number from a reference. In the 2nd paragraph under the section “Global behavior of RNA degradation”, [they] mentioned that the average RNA lifetime in E.coli reported by previous papers is 6 min. This is incorrect: the average half‐life, not lifetime, is ~6 min (lifetime equivalent is ~8 min)." |
| Entered by |
Uri M |
| ID |
112326 |