| Range |
Table - link µm^2/sec
|
| Organism |
Bacteria Lactococcus lactis |
| Reference |
Mika JT, Schavemaker PE, Krasnikov V, Poolman B. Impact of osmotic stress on protein diffusion in Lactococcus lactis. Mol Microbiol. 2014 Nov94(4):857-70. doi: 10.1111/mmi.12800. Supplementary information table S3PubMed ID25244659
|
| Method |
FRAP [fluorescent recovery after photobleaching] |
| Comments |
P.858 right column bottom paragraph:"For membrane proteins FRAP was performed in the same way as for the cytosolic proteins (Fig. 1B). For the GFP-tagged membrane proteins LacSΔIIA and BcaP, [investigators] determined the following diffusion coefficients: for LacSΔIIA-GFP Dmean = 0.020 μm^2 s^−1 and for BcaP-GFP Dmean = 0.019 μm^2 s^−1 (Fig. 3A, Table S3, n ≥ 22 cells), which is much slower than the Dmedian of GFP or β-galactosidase-GFP in the cytoplasm of L. lactis. These values are similar to those obtained for membrane proteins of similar size expressed in the cytoplasmic membrane of E. coli, 0.017–0.028 μm^2 s^−1 (Kumar et al., 2010)." See notes beneath table |
| Entered by |
Uri M |
| ID |
112271 |