| Comments |
P.143 right column bottom paragraph:"Then, in order to rule out the possibilities that the apparent difference of measured transporting velocities between the initial and later (more than 1 hr after injection) time points might reflect any different mechanism of transport, [investigators] repeated the same kind of pharmacological experiments with creatine kinase using the long time points (Table 2). [They] measured the transporting distance of creatine kinase from injection point after 1, 2, and 3 hr postinjection. During 1 and 2 hr postinjection, [they] applied nocodazole (at the concentration of 40 μg/ml), or cytochalasin D (at the concentration of 2 μM) to the bath. As shown in Table 2, the first transporting distance per hour was not different from control specimen (control, 128.7 ± 91.1 μm [n = 3] nocodazole treatment, 127.6 ± 55.5 μm [n = 3] cytochalasin D treatment, 135.5 ± 97.3 [n = 3]), but only nocodazole treatment profoundly inhibited transport during 1 and 2 hr postinjection (control, 369.9 ± 39.1 μm [n = 3] nocodazole treatment, 222.8 ± 77.4 μm [n = 3] cytochalasin D treatment, 326.3 ± 69.7 [n = 3])." Animals studied were Heterololigo bleekeri, Todarodes pacificus pacificus, or Loligo pealei. |