| Range |
Figure - link sec
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| Organism |
Monkey |
| Reference |
Loerke D et al., Cargo and dynamin regulate clathrin-coated pit maturation. PLoS Biol. 2009 Mar 17 7(3):e57. doi: 10.1371/journal.pbio.1000057. p.0636 figure 6PubMed ID19296720
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| Method |
P.0637 left column 2nd paragraph:"TIR-FM [Total internal reflection fluorescence microscopy] was performed on BSC1 monkey kidney epithelial cells stably expressing rat brain clathrin LCa-EGFP or the AP2 rat brain σ2-adaptin fused to EGFP (provided by Dr. T. Kirchhausen, Harvard Medical School, and described in [ref 2]) using a 100 × 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon)." |
| Comments |
P.0636 right column 2nd paragraph:"A model describing these results is illustrated in Figure 6. In this model, productive CCP [clathrin-coated pit] formation is a stochastic event initiated by the cargo-independent association of AP2 [AP2 adaptor] at the plasma membrane, which nucleates clathrin assembly. If a critical mass of the additional factors required for CCP stabilization is not reached during this assembly phase, these structures, which correspond to early and late abortive CCPs, fail to pass through the restriction point and disassemble." See note beneath figure |
| Entered by |
Uri M |
| ID |
112143 |