| Range |
two short-lived subpopulations 5.2±0.1s and 15.9±1s: long-lived subpopulation 86.9±5.8s: average 39s sec
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| Organism |
Monkey |
| Reference |
Loerke D et al., Cargo and dynamin regulate clathrin-coated pit maturation. PLoS Biol. 2009 Mar 17 7(3):e57. doi: 10.1371/journal.pbio.1000057. p.0629 right column 2nd paragraph & 3rd paragraph & p.0635 left column bottom paragraphPubMed ID19296720
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| Method |
P.0637 left column 2nd paragraph:"TIR-FM [Total internal reflection fluorescence microscopy] was performed on BSC1 monkey kidney epithelial cells stably expressing rat brain clathrin LCa-EGFP or the AP2 [AP2 adaptor] rat brain σ2-adaptin fused to EGFP (provided by Dr. T. Kirchhausen, Harvard Medical School, and described in [ref 2]) using a 100 × 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon)." |
| Comments |
p.0629 right column 2nd paragraph:"[Investigators’] data contained two short-lived subpopulations with time constants of 5.2 ± 0.1 s and 15.9 ± 1 s, respectively (±jackknifed cell-to-cell error, see Table S1) that were best fit with Rayleigh distributions, i.e., the shortest and longest lifetimes within the population occur less frequently than the intermediate ones. This suggests that [investigators’] time sampling of 0.4 s per frame was sufficient to capture all events of significant clathrin coat accumulation. A single longer-lived subpopulation (time constant 86.9 ± 5.8 s) was best approximated by an exponentially decaying distribution." p.0629 right column 3rd paragraph:"Taking into account the different mean lifetimes and relative contributions of the three individual subpopulations, the mean lifetime of all CCPs is 39 s, much shorter than the half-time of transferrin (Tfn) uptake. This further supports the hypothesis that the two short-lived subpopulations are abortive and do not contribute to Tfn uptake." |
| Entered by |
Uri M |
| ID |
112142 |