Diffusion coefficient of G-actin in cytoplasm
| Range | 3 - 30 μm^2/s |
|---|---|
| Organism | Mammals |
| Reference | Kiuchi T, Nagai T, Ohashi K, Mizuno K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension. J Cell Biol. 2011 Apr 18 193(2):365-80. doi: 10.1083/jcb.201101035. p.368 left column top paragraphPubMed ID21502360 |
| Primary Source | McGrath, J.L., Y. Tardy, C.F. Dewey Jr., J.J. Meister, and J.H. Hartwig. 1998. Simultaneous measurements of actin filament turnover, filament fraction, and monomer diffusion in endothelial cells. Biophys. J. 75: 2070–2078. doi:10.1016/S0006-3495(98)77649-0 & Zicha, D., I.M. Dobbie, M.R. Holt, J. Monypenny, D.Y.H. Soong, C. Gray, and G.A. Dunn. 2003. Rapid actin transport during cell protrusion. Science. 300:142–145. doi:10.1126/science.1082026 & McDonald, D., G. Carrero, C. Andrin, G. de Vries, and M.J. Hendzel. 2006. Nucleoplasmic beta-actin exists in a dynamic equilibrium between lowmobility polymeric species and rapidly diffusing populations. J. Cell Biol. 172:541–552. doi:10.1083/jcb.200507101PubMed ID9746549, 12677069, 16476775 |
| Comments | P.367 right column:"...The diffusion coefficient (13.7 μm^2/s) was consistent with the reported values for G-actin in the cytoplasm, which ranged from 3 to 30 μm^2/s (primary sources). These results indicate that single FDAP [fluorescence decay after photoactivation] analysis is a useful tool for estimating G-actin concentration in living cells. Based on the half-life of the mobile fraction (41 ms), [investigators] set the time point for image acquisition for s-FDAP analysis as 40ms after photoactivation." 1st primary source studied subconfluent bovine aortic endothelial cells (BAECs). 2nd primary source studied rat fibroblasts. 3rd primary source studied HeLa cells. |
| Entered by | Uri M |
| ID | 112133 |