Synthesis and degradation rate constant of 3OC6-HSL (3OC6-homo-serine lactone) in the lux system

Range synthesis rate 3,300nM/hr: degradation rate 0.108/hour
Organism Bacteria Vibrio fischeri
Reference Pai A, You L: Optimal tuning of bacterial sensing potential. Molecular systems biology 2009, 5(286) :286 doi: 10.1038/msb.2009.43. Supplementary Text 2 p.26 bottom paragraphPubMed ID19584835
Primary Source Kaufmann GF et al., Revisiting quorum sensing: Discovery of additional chemical and biological functions for 3-oxo-N-acylhomoserine lactones. Proc Natl Acad Sci U S A. 2005 Jan 11 102(2):309-14. AND Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Jr., Greenberg EP (1996) Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein. Proceedings of the National Academy of Sciences of the United States of America 93: 9505-9509.PubMed ID15623555, 8790360
Comments Supplementary Text 2 p.26 bottom paragraph:"lux system (Type II) - In the lux system, 3OC6-HSL induces luminescence at high cell densities by binding to the intracellular transcriptional regulator LuxR (Fuqua et al., 1996 Kaplan & Greenberg, 1985). Synthesis rate: The turnover rate of LuxI synthase (primary source Schaefer et al., 1996) is 1.1 mol of 3OC6-HSL min^-1 mol^-1 LuxI synthase, giving k = 3.3×10^3 nM/hr. Degradation rate constant: From the hydrolysis rate (primary source Kaufmann et al., 2005) of 3OC6-HSL da=3.07×10^-5s^-1 = 0.108 hr^-1."
Entered by Uri M
ID 112005