Biotin - streptavidin binding force

Value 257 pN Range: ±25 pN
Organism Generic
Reference VT. Moy, EL. Florin, HE. Gaub, Intermolecular forces and energies between ligands and receptors. Science. 1994 Oct 14 266(5183):257-9. p.257 middle column 2nd paragraph & fig.1A Table - link PubMed ID7939660
Method AFM (atomic force microscopy, scan mode)
Comments p.257 middle column 2nd paragraph:"The Unbinding force of five different avidin-biotin pairs varied from 85±10pN for avidin-iminobiotin to 257±25 pN for streptavidin-biotin (Fig. 1A)."
Entered by JoeG
ID 111925