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Doubling times in nutrient-poor (M2G) or nutrient-rich (PYE) medium at 30°C

Range	nutrient poor 146±5: nutrient-rich 96±1 min
Organism	Bacteria Caulobacter crescentus
Reference	Campos M et al. (2014) A constant size extension drives bacterial cell size homeostasis. Cell 159:1433–46. doi: 10.1016/j.cell.2014.11.022. p.1434 left column bottom paragraphPubMed ID25480302
Method	p.1434 left column 4th paragraph:"In this study, [investigators] performed high-precision single-cell timelapse microscopy studies on C. crescentus and E. coli to uncover the intrinsic principles of bacterial cell size homeostasis for both symmetric and asymmetric divisions."
Comments	p.1434 left column bottom paragraph:"There was no discernable difference in cell length distributions between C. crescentus populations grown in nutrient-poor (M2G) or nutrient-rich (PYE) medium (Figure 1B) despite significant differences in growth rates, with doubling times of 146 ± 5 min in M2G (mean ± SD, n = 2 experiments) and 96 ± 1 min in PYE (n = 2). Varying cell density (OD660nm < 0.3 versus > 0.8) or temperature (25°C versus 30°C) also had little effect on cell length distributions (Figure 1B)." p.1444 right column 3rd paragraph:"PYE medium = (2 g/l bacto-peptone, 1 g/l yeast extract, 1 mM MgSO4, 0.5 mM CaCl2). M2G medium = (0.87 g/l Na2HPO4, 0.54 g/l KH2PO4, 0.50 g/l NH4Cl, 0.2% (w/v) glucose, 0.5 mM MgSO4, 0.5mM CaCl2, 0.01 mM FeSO4)."
Entered by	Uri M
ID	111766