Doubling time in M9-supplemented medium

Value 42 min Range: ±12 min
Organism Bacteria Escherichia coli
Reference Campos M et al. (2014) A constant size extension drives bacterial cell size homeostasis. Cell 159:1433–46. doi: 10.1016/j.cell.2014.11.022. p.1439 right column top paragraphPubMed ID25480302
Method p.1434 left column 4th paragraph:"In this study, [investigators] performed high-precision single-cell timelapse microscopy studies on C. crescentus and E. coli to uncover the intrinsic principles of bacterial cell size homeostasis for both symmetric and asymmetric divisions."
Comments p.1439 left column bottom paragraph:"It is well known that E. coli changes its size in response to nutrient availability (Schaechter et al., 1958). If cell size homeostasis works through a constant elongation irrespective of the composition of the growth medium, [investigators] would expect the fixed cell length extension in nutrient-poor medium to be smaller than in the nutrient-rich LB medium, with the average ?L value matching the average Lb value for each growth medium. To test this hypothesis, [they] grew E. coli BW25113 in microfluidic chambers with M9-supplemented medium (M9 salts supplemented with 0.1% casamino acids and 0.2% glucose) instead of LB medium. Under steady-growth conditions (Figure S4), the cell-cycle time in M9 supplemented medium was longer than in LB medium with T = 42 ± 12 min, and the cell lengths at birth and at division were shifted to lower values (Figure 5A), with Lb = 2.32 ± 0.38 µm and Ld = 4.59 ± 0.71 µm. Cell size control in M9 medium remains precise, with CV values of 17% and 15% for Lb and Ld, respectively." p.1444 right column 3rd paragraph:"M9-supplemented medium=(6 g/l Na2HPO4·7H2O, 3 g/l KH2PO4, 0.5 g/l NaCl, 1 g NH4Cl, 2 mM MgSO4, 1 mg/l thiamine supplemented with 0.1% casamino acids and 0.2% glucose)."
Entered by Uri M
ID 111767