| Comments |
p.209 left column 2nd paragraph:"Measurements of the cellular composition are necessary to structure the flux of carbon to biomass, and especially if the cellular composition changes with the operating conditions it is important to consider the differences in fluxes to the different macromolecular pools. In this study, the cellular composition was therefore determined at four different dilution rates (see Table 4). The most important variation in the cellular composition is that the amount of active machinery, i.e. protein and RNA, increases linearly with increasing dilution rate at the expense of carbohydrates. The cellular content of other components is virtually independent of the dilution rate. From Table 4 it is seen that the measurements can account for approximately 100% of the cell mass, but since the pool of e.g. glycolytic intermediates has not been measured, some of the analyses have a small overlap. However, the results indicate that no major cellular component has been left out. The values listed in Table 4 are in good accordance with previously reported values for the cellular composition of S. cerevisiae (Kuenzi & Fiechter, 1972 Oura, 1972 Watson, 1976 Waldron, 1977 Furukawa et al., 1983 Verduyn et al., 1990)." |