| Comments |
"The parallel measurement of H+/ATP for both MF0F1 and CF0F1 under identical experimental conditions in this high-precision system ensured that the n values obtained for both enzymes can be directly compared, in particular the imposed ?uH+ values, the stoichiometric product Q, and the ?G°'p were identical for both enzymes. [Investigators’] results were H+/ATP = 2.9 ± 0.2 for MF0F1 and H+/ATP = 3.9 ± 0.3 for CF0F1. The CF0F1 value is in agreement with the value 4.0 (± 0.2) reported earlier (refs 18, 19, 25, 26). As to MF0F1, different numbers, mainly 2 or 3, had been reported for the mammalian enzyme over the past decades (refs 27, 28 for review see ref. 29), but no data had been published for the yeast MF0F1." CF0F1 from chloroplasts: MF0F1 from yeast mitochondria |