| Range |
Table - link
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Taymaz-Nikerel H, Borujeni AE, Verheijen PJ, Heijnen JJ, van Gulik WM. Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry. Biotechnol Bioeng. 2010 Oct 1 107(2):369-81. doi: 10.1002/bit.22802. p.375 table VPubMed ID20506321
|
| Primary Source |
Emmerling M et al., Metabolic flux responses to pyruvate kinase knockout in Escherichia coli. J Bacteriol. 2002 Jan184(1):152-64. & Hua Q, Yang C, Baba T, Mori H, Shimizu K. Responses of the central metabolism in Escherichia coli to phosphoglucose isomerase and glucose-6-phosphate dehydrogenase knockouts. J Bacteriol. 2003 Dec185(24):7053-67. & Fischer E, Sauer U. A novel metabolic cycle catalyzes glucose oxidation and anaplerosis in hungry Escherichia coli. J Biol Chem. 2003 Nov 21 278(47):46446-51.PubMed ID11741855, 14645264, 12963713
|
| Method |
Abstract:"The unknown ATP stoichiometry parameters of the constructed E. coli network were estimated from experimental data of eight different aerobic chemostat experiments carried out with E. coli MG1655, grown at different dilution rates (0.025, 0.05, 0.1, and 0.3 h(-1)) and on different carbon substrates (glucose, glycerol, and acetate)." |
| Comments |
"The macromolecular composition of the biomass is known
to change with environmental conditions and specific growth
rate (Bremer and Dennis, 1996). However, [investigators’] measurements
of the protein content of E. coli cells grown in glucose limited
chemostats showed that the protein content did not change
significantly with changes in specific growth rate. The measured protein content agreed well with published data
(Table V).
Unfortunately the performed RNA measurements did
not yield reproducible results: therefore published data
(Table V), yielding a linear relation between RNA content
and specific growth rate (Emmerling et al., 2002), were used
to calculate the RNA content as a function of the specific
growth rate." |
| Entered by |
Uri M |
| ID |
111462 |