| Value |
69
min
Range: 45 - 114 min
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Hebisch E, Knebel J, Landsberg J, Frey E, Leisner M. High variation of fluorescence protein maturation times in closely related Escherichia coli strains. PLoS One. 2013 Oct 14 8(10):e75991. doi: 10.1371/journal.pone.0075991. p.6 right column top paragraphPubMed ID24155882
|
| Method |
"Here, [investigators] analyzed the maturation kinetics of the fluorescent protein mCherry. As a
derivative of DsRed [ref 18 PMID 15558047], mCherry chromophore formation has to
undergo two subsequent oxidation steps [ref 29 PMID 16893165]. The first oxidation
step creates a green intermediate [ref 28 PMID 10504696], and the second oxidation
step leads to an increase in conjugated double bonds. As a
consequence, one observes a shift to longer absorbance and
emission wavelengths [ref 28 PMID 10504696]. [Investigators] measured both the temporal
fluorescence development of the green intermediate and of the red
fluorescent state of the final mCherry protein in parallel (Figure
S5). [They] obtained the characteristic sigmoidal fluorescence
development for the red fluorescent protein after CAP addition." |
| Comments |
"Whereas maturation kinetics of the
mCherry ancestor DsRed was theoretically described by a threestep
process [ref 25 PMID 11812239], [investigators’] experimentally observed data for mCherry
maturation kinetic was adequately described by a two-step process
with an additional back reaction from the red fluorescent state to
the green fluorescent state (Supporting Information S1). The
average mCherry maturation time of the S, R and C strain was
69 min, with a high variation ranging from 45 to 114 min. [Investigators’]
three E. coli strains showed significantly longer mCherry maturation
times than for example E. coli LMG194 with 15 min [ref 18 PMID 15558047] or
PAmCherrys expressed in E. coli LMG194 with 18 to 25 min [ref 19 PMID 19169259]." |
| Entered by |
Uri M |
| ID |
111423 |