Difference between in vivo & in vitro measurements of kinetic parameters

Range ≤3 orders of magnitude
Organism Generic
Reference Bar-Even A. et al., The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters. Biochemistry. 2011 May 31 50(21):4402-10. doi: 10.1021/bi2002289. p.4403 right column top paragraphPubMed ID21506553
Primary Source [32] Wright, B. E., Butler, M. H., and Albe, K. R. (1992) Systems analysis of the tricarboxylic acid cycle in Dictyostelium discoideum . I. The basis for model construction. J. Biol. Chem. 267, 3101 – 3105.PubMed ID1737766
Method "Here [investigators] describe a global view of enzyme parameters with the aim of highlighting the forces that shape the catalytic efficiency of enzymes."
Comments "Further, most parameters in the literature are measured in vitro, which can be considerably different from those measured in vivo. [refs 32,33] These differences can be as great as 3 orders of magnitude. [primary source]"
Entered by Uri M
ID 111410