| Value |
9
nm
Range: ±1.4 nm
|
| Organism |
Chlamydomonas reinhardtii |
| Reference |
Engel, B. D., Schaffer, M., Kuhn Cuellar, L., Villa, E., Plitzko, J. M., and Baumeister, W. (2015). Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography. Elife 4, 1–29. p.4 bottom paragraph & p.14 2nd paragraphPubMed ID25584625
|
| Method |
in situ cryo-electron tomography |
| Comments |
P.4 bottom paragraph: "Throughout the chloroplast, thylakoids were most commonly found in stacks of variable length but uniform spacing. Thylakoid stacks had a lateral repeat distance of 22.4 ± 1.3 nm, with 4.9 ± 0.5 nm thylakoid membranes, 9.0 ± 1.4 nm thylakoid lumina, and 3.6 ± 0.5 nm interthylakoid stromal spaces (± denotes standard deviation, n = 38 thylakoids from eight stacks)." P.14 2nd paragraph: "[Researchers'] Chlamydomonas cells, which were grown in constant light until the moment of vitrification, had 9 nm thylakoid
lumina. Interestingly, this may be a result of light conditions rather than an algal characteristic, as
leaves that were harvested from light-exposed plants and immediately cryo-immobilized had 9.2 nm grana lumina (Kirchhoff et al., 2011)." |
| Entered by |
Avi Flamholz |
| ID |
111326 |