Diameter of cylindrical pyrenoid tubules in chloroplast

Value 107 nm Range: ±26 nm
Organism Chlamydomonas reinhardtii
Reference Engel, B. D., Schaffer, M., Kuhn Cuellar, L., Villa, E., Plitzko, J. M., and Baumeister, W. (2015). Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography. Elife 4, 1–29. p.9 top paragraphPubMed ID25584625
Method in situ cryo-electron tomography
Comments "Membrane tubules extend from the thylakoids into the pyrenoid through fenestrations in the starch sheath (Sager and Palade, 1957 Ohad et al., 1967b). [Researchers] aimed to better understand the function of these intriguing structures by visualizing their native 3D architecture (Figures 9–11). Thylakoid stacks often changed direction (Figure 9A–D) as their sheets merged with each other and narrowed into cylindrical pyrenoid tubules (Figure 10), which had a mean diameter of 107 ± 26 nm (n = 46 tubules from 10 tomograms). A 5–10 nm RuBisCO-free space was frequently observed surrounding the tubules (Figure 9E,G)."
Entered by Uri M
ID 111331