| Comments |
"The Ig domains of
DdFLN [shortened actin crosslinker dictyostelium
filamin] exhibit unfolding forces in a range between 40 and 100 pN
(5), and GFP unfolding in this chimera construct should occur at
higher extensions after the weakest Ig domains have already
unfolded. In force extension traces obtained with DdFLN1-5–GFP
(Fig. 1d), the GFP unfolding events are indeed shifted toward
higher extensions. Nonetheless, both the length gain and the
unfolding force are identical to Ig8–GFP. Results for the two
constructs are therefore in agreement, and [researchers] conclude that at
pulling velocities of 300nm/s GFP on average can bear mechanical
loads of 104±40 pN (Fig. 1e) before it unfolds." |