| Method |
"Hydrogen-bonding criteria were derived from small molecule
crystal studies and are similar to those used in
an earlier study (Presta & Rose, 1988). For each protein,
hydrogen bonds were identified in a 3-step process...Second, distances between all potential donor-acceptor
pairs in the protein were evaluated. A pair was retained
for further analysis whenever its inter-atomic distance was
less than or equal to the sum of the hydrogen bond radii
of its respective atoms. It should be noted that the
hydrogen bond radii in Table 1 are about 10% larger than
the corresponding van der Waals’ radii." |