Coenzyme & adenine nucleotide levels in vegetative cells and dormant spores

Range Table - link nmoles/g, wet weight
Organism Bacteria Bacillus spp.
Reference Setlow, P., and Kornberg, A. (1970). Biochemical studies of bacterial sporulation and germination. XXII. Energy metabolism in early stages of germination of Bacillus megaterium spores. J. Biol. Chem. 245, 3637–3644. p.3639 tables I & IIPubMed ID4394282
Method "Germinating spores were extracted with the boiling propanol procedure described above, but without the added phosphate. All adenine nucleotides are extracted from the spores in 5 min, and there is little or no ATP breakdown (19)."
Comments "Coenzyme Levels-The levels of FAD, DPN+, and TPN+ found in the dormant spore were similar to those in cells at midlog phase (Table I). The latter values agree with those reported in other strains of B. megaterium (26, 31). Whereas the ratio of reduced to oxidized pyridine nucleotides in bacterial cells is in the range of 0.14 to 0.30 (32, 33), that in dormant spores was extremely low (0.02 or less). This ratio, as well as the levels of coenzymes, was not affected by storage for 5 months in the lyophilized state at room temperature. Adenine Nucleotide Levels-The level of total adenine nucleotides, as with the coenzyme levels, was similar in spores and cells (Table II) (ref 1). Strikingly different was the ATP charge (ratio of ATP plus 0.5 ADP to total adenine nucleotide (34)). The value was 0.1 in spores as compared to 0.8 to 0.9 in cells. The trace of nucleotide determined as ATP in dormant spores by the luciferase assay was further identified as ATP by its removal upon treatment of the spore extract with glucose and hexokinase." See notes beneath tables
Entered by Uri M
ID 111170