| Range |
Table - link nmoles/g, wet weight
|
| Organism |
Bacteria Bacillus spp. |
| Reference |
Setlow, P., and Kornberg, A. (1970). Biochemical studies of bacterial sporulation
and germination. XXII. Energy metabolism in early stages of germination of
Bacillus megaterium spores. J. Biol. Chem. 245, 3637–3644. p.3639 tables I & IIPubMed ID4394282
|
| Method |
"Germinating spores were extracted with the boiling propanol
procedure described above, but without the added phosphate.
All adenine nucleotides are extracted from the spores in 5 min,
and there is little or no ATP breakdown (19)." |
| Comments |
"Coenzyme Levels-The levels of FAD, DPN+, and TPN+ found
in the dormant spore were similar to those in cells at midlog
phase (Table I). The latter values agree with those reported
in other strains of B. megaterium (26, 31). Whereas the ratio of
reduced to oxidized pyridine nucleotides in bacterial cells is in
the range of 0.14 to 0.30 (32, 33), that in dormant spores was
extremely low (0.02 or less). This ratio, as well as the levels of
coenzymes, was not affected by storage for 5 months in the
lyophilized state at room temperature.
Adenine Nucleotide Levels-The level of total adenine nucleotides,
as with the coenzyme levels, was similar in spores and cells (Table II) (ref 1). Strikingly different was the ATP charge (ratio of
ATP plus 0.5 ADP to total adenine nucleotide (34)). The value
was 0.1 in spores as compared to 0.8 to 0.9 in cells. The trace of
nucleotide determined as ATP in dormant spores by the luciferase
assay was further identified as ATP by its removal upon
treatment of the spore extract with glucose and hexokinase." See notes beneath tables |
| Entered by |
Uri M |
| ID |
111170 |