Transcription rate of dystrophin mRNA

Range ~2.4 (1.7 - 2.5) kbp/min
Organism Human Homo sapiens
Reference Jackson DA, Pombo A, Iborra F. The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 2000 Feb14(2):242-54. p.243 left column 3rd paragraphPubMed ID10657981
Primary Source [24] Tennyson, C. N., Klamut, H. J., and Worton, R. G. (1995) The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced. Nat. Genet. 9, 184–190 p.187 right column top paragraphPubMed ID7719347
Method Primary source abstract: "Quantitative reverse transcription-polymerase chain reaction (RT-PCR) results indicate that approximately 12 h are required for transcription of 1,770 kb (at an average elongation rate of 2.4 kb/min), extrapolating to a transcription time of 16 h for the complete gene."
Comments P.243 left column 3rd paragraph: "Estimates of the rate of synthesis by pol II rely on the time of appearance of sequences located at known distances along a transcript. In human cells, quantitative polymerase chain reaction (PCR) was used to analyze transcription across different regions of the ~2.5 Mb dystrophin gene following the induction of expression in muscle cell cultures. Elongation rates in the range 1.7–2.5 kb/min were determined (primary source)." Primary source p.187 right column top paragraph: "The rate of elongation ranges from 100-151kb/hour or 1.7-2.5kb/min, consistent with the value of 2.4kb/min measured in the previous experiment."
Entered by Uri M
ID 111156