| Range |
~2.4 (1.7 - 2.5) kbp/min
|
| Organism |
Human Homo sapiens |
| Reference |
Jackson DA, Pombo A, Iborra F. The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 2000 Feb14(2):242-54. p.243 left column 3rd paragraphPubMed ID10657981
|
| Primary Source |
[24] Tennyson, C. N., Klamut, H. J., and Worton, R. G. (1995) The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced. Nat. Genet. 9, 184–190 p.187 right column top paragraphPubMed ID7719347
|
| Method |
Primary source abstract: "Quantitative reverse transcription-polymerase chain reaction (RT-PCR) results indicate that approximately 12 h are required for transcription of 1,770 kb (at an average elongation rate of 2.4 kb/min), extrapolating to a transcription time of 16 h for the complete gene." |
| Comments |
P.243 left column 3rd paragraph: "Estimates of the rate of synthesis by pol II rely on the time of appearance of sequences located at known distances along a transcript. In human cells,
quantitative polymerase chain reaction (PCR) was used to analyze transcription across different regions of the ~2.5 Mb dystrophin gene following the induction
of expression in muscle cell cultures. Elongation rates in the range 1.7–2.5 kb/min were determined (primary source)." Primary source p.187 right column top paragraph: "The rate of elongation ranges from 100-151kb/hour or 1.7-2.5kb/min, consistent with the value of 2.4kb/min measured in the previous experiment." |
| Entered by |
Uri M |
| ID |
111156 |