| Method |
"Junctional acetylcholine receptor site densities ([125I]a-BTX binding
site densities) were determined for developing endplates in EDL [Extensor digitorum longus] muscles
at 1, 3, 5, and 14 days after birth and for adults (>3 months). All
animals were of strain 129/ReJ: the number of animals sampled at each
age is indicated in parentheses. All of the immature muscles and three
of the five adult muscles were labeled after prefixation. Junctional site
densities (mean and standard error) are expressed both as sites/µm^2 of
junctional surface (sites/µm^2 1° cleft midline) and as sites/µm^2 of
specialized thickened membrane. Since, as indicated, at least 200 autoradiographic
grains were collected for each analysis, the overall autoradiographic sampling error 100/(N^0.5) at each stage was always <10%." |