| Range |
for dark-adapted cells 3.1±0.5×10^-1: after bleach 2.0±0.2×10^-3 pA/photon×µm^2
|
| Organism |
Mouse Mus musculus |
| Reference |
Wang JS, Kefalov VJ. An alternative pathway mediates the mouse and human cone visual cycle. Curr Biol. 2009 Oct 13 19(19):1665-9. doi: 10.1016/j.cub.2009.07.054. p.1665 left column bottom paragraphPubMed ID19781940
|
| Method |
"[Researchers] measured flash sensitivity of single photoreceptors before and after exposure to bright light to determine whether the mouse retina, isolated from the pigment epithelium, is able to promote cone pigment regeneration and dark adaptation. [Researchers] used a suction electrode to record membrane current from individual mouse rods and cones in retina removed from the pigment epithelium. Rod recordings were done from wild-type (WT C57BL/6) mice, and cone recordings were done from rod transducin a-subunit knockout (Tra-/-) mice, as described previously [ref 9]." |
| Comments |
"[Researchers] compared the dark current and sensitivity of photoreceptors before (dark adapted) and after 40 s of exposure to bright light followed by recovery in darkness (bleached, DA). With subsequent 4 hr dark incubation, exposure of rods in
isolated retina to bleaching light produced a significant decrease in dark current, from 15 pA to 5 pA, and a 155-fold decrease in their flash sensitivity, from 3.1±0.5×10^-1 (n = 6)
pA/photon×µm^2 for dark-adapted cells to 2.0±0.2×10^-3 (n = 6) pA/photon×µm^2 after bleach (Figure 1A)." |
| Entered by |
Uri M |
| ID |
110984 |