| Range |
Table - link mM
|
| Organism |
Plasmodium falciparum |
| Reference |
Teng R, Junankar P, Bubb W, Rae C, Mercier P, et al. (2009) Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by 1H NMR spectroscopy. NMR Biomed 22: 292-302 DOI:10.1002/nbm.1323 pp.296-7 table 1PubMed ID19021153
|
| Method |
"[Researchers] used 1H NMR spectroscopy to analyse the
metabolome of mature, trophozoite-stage P. falciparum parasites, ‘isolated’ from the contents of their host erythrocytes by
saponin-permeabilisation of the host erythrocyte membrane. Although it is recognised that the process of isolating parasites
from their host cell will invariably lead to some loss of metabolites
from the parasite (and hence to an underestimate of the
intracellular concentrations of some solutes), the aim here was
to identify parasite-specific compounds, as distinct from host cell
metabolites." |
| Comments |
"Table 1 shows the estimated intracellular concentrations of
41 compounds or, in the case of the pyrimidine nucleotides,
combinations of compounds, detected by 1H NMR in the different
extracts of isolated P. falciparum trophozoites. For a number of
other metabolites detected (e.g. pyruvate, citrate, hypoxanthine,
IMP, NADP+, glycerophosphocholine and glycerophosphoethanolamine),
the NMR peaks overlapped and/or were not
sufficiently distinct to permit quantification." See notes beneath table |
| Entered by |
Uri M |
| ID |
110870 |