| Value |
99
%
Range: ±16 %
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Shearer G, Lee JC, Koo JA, Kohl DH. Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli. FEBS J. 2005 Jul272(13):3260-9. p.3262 left column top paragraphPubMed ID15978033
|
| Method |
"In this study, [researchers] describe experiments aimed at calculating
the fraction of the flux from early glycolytic
intermediates to CO2 in intact Escherichia coli. Cells
incubated with [14C]glucose made 14C-labeled glycolytic
intermediates. When the incubation mix also
included a [12C]intermediate, assuming that this intermediate
entered the cell, there was a competition
between the intermediate just produced in the pathway
and the same molecular species in the aqueous cytosol.
To the degree the latter was successful, the amount of
14CO2 evolved was decreased. Cells were also incubated
in [12C]glucose plus [14C]intermediate, [14C]glucose
alone and [14C]intermediate alone. Combining these
data allowed [them] to calculate the fraction of the total
flux to CO2 that was channeled. [They] used an E. coli mutant that was engineered to take up 6-carbon sugar
phosphates constitutively." |
| Comments |
"The results indicate a high
degree of channeling of fructose 1,6-bisphosphate
(Fru1,6P2) to CO2
(99±16% of the flux from
Fru1,6P2 channeled, P=0.005). This result requires
that all of the downstream steps from Fru1,6P2 to
pyruvate and the further oxidation of pyruvate to CO2
(presumably via mixed acid fermentation) be 100%
channeled." |
| Entered by |
Uri M |
| ID |
110834 |