| Range |
Table - link
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Shearer G, Lee JC, Koo JA, Kohl DH. Quantitative estimation of channeling from early glycolytic intermediates to CO in intact Escherichia coli. FEBS J. 2005 Jul272(13):3260-9. p.3263 table 2PubMed ID15978033
|
| Method |
"In this study, [researchers] describe experiments aimed at calculating
the fraction of the flux from early glycolytic
intermediates to CO2 in intact Escherichia coli. Cells
incubated with [14C]glucose made 14C-labeled glycolytic
intermediates. When the incubation mix also
included a [12C]intermediate, assuming that this intermediate
entered the cell, there was a competition
between the intermediate just produced in the pathway
and the same molecular species in the aqueous cytosol.
To the degree the latter was successful, the amount of
14CO2 evolved was decreased. Cells were also incubated
in [12C]glucose plus [14C]intermediate, [14C]glucose
alone and [14C]intermediate alone. Combining these
data allowed [them] to calculate the fraction of the total
flux to CO2 that was channeled. [They] used an E. coli mutant that was engineered to take up 6-carbon sugar
phosphates constitutively." |
| Comments |
"Using the data shown in Table 2 and calculating as
described in Experimental procedures, about 96% of
the CO2 was evolved via oxidation of pyruvate followed
by mixed acid fermentation and the TCA cycle.
Therefore, [researchers] have ignored the CO2 evolved by the
OPPP when making calculations of the fractional
importance of channeled flux in glycolysis." |
| Entered by |
Uri M |
| ID |
110837 |