Phloretin inhibited urea permeability of red cells
| Range | ~1E-6 cm/sec |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Brahm J. Urea permeability of human red cells. J Gen Physiol. 1983 Jul82(1):1-23. p.2 2nd paragraphPubMed ID6411854 |
| Method | "The present study was undertaken to provide a more detailed characterization of urea transport kinetics in human red cells. The results show that urea transport has features that are commonly applied in characterizing a facilitated diffusion process: saturation kinetics of the Michaelis-Menten type, competitive inhibition with the urea analogue thiourea, and irreversible inhibition with p-chloromercuribenzosulfonate (PCMBS)." |
| Comments | "Phloretin inhibited urea permeability of human red cells by two orders of magnitude to ~1X10^-6cm/sec (at room temperature)(note that value in article is "~1X10^6", in error) . This is as low as urea permeability in artificial lipid membranes (Vreeman, 1966 Galucci et al ., 1971 Poznansky et al., 1976 Finkelstein, 1976) and in the chicken erythrocyte membrane (Brahm and Wieth, 1977), systems where urea permeates by simple diffusion." |
| Entered by | Uri M |
| ID | 110727 |