Reduction in T cell numbers when treated with the antimitotic drug hydroxyurea

Value 50 %
Organism Mouse Mus musculus
Reference Tough DF, Sprent J. Life span of naive and memory T cells. Stem Cells. 1995 May13(3):242-9. p.243 right column top paragraphPubMed ID7613491
Primary Source Rocha B, Freitas AA, Coutinho AA. Population dynamics of T lymphocytes. Renewal rate and expansion in the peripheral lymphoid organs. J Immunol. 1983 Nov131(5):2158-64.PubMed ID6605376
Method (Primary source abstract:) "T cell subpopulations in the thymus, bone marrow, spleen, and lymph nodes of normal, shamthymectomized, thymectomized, and splenectomized + thymectomized BALB/c or C57BL/6 mice were identified with monoclonal antibodies to Thy-1, Lyt-1, and Lyt-2 antigens at different times after the administration of hydroxyurea in a regimen shown to deplete cycling cells."
Comments "In one study, treatment of mice with the antimitotic drug hydroxyurea, which selectively kills cycling cells, reduced T cell numbers by 50% within 2 to 3 days (primary source)."
Entered by Uri M
ID 110716