Viral reads classification
Range | Table - link |
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Organism | Virus |
Reference | Popgeorgiev N et al., Marseillevirus-like virus recovered from blood donated by asymptomatic humans. J Infect Dis. 2013 Oct 1 208(7):1042-50. doi: 10.1093/infdis/jit292.PubMed ID23821720 |
Method | "Pyrosequencing DNA extracted from the viral-enriched fraction of the blood of asymptomatic blood donors generated 20,238 reads (NCBI accession number: PRJNA183996), which were classified by their taxonomic distribution (Figure 1). A best BLAST hits comparison against the GenBank database (BLASTn E value, <10^-5) indicated that 17,257 metagenomic reads (85.3%) had significant hits and that 67.7% of these reads were related to viruses." |
Comments | "The majority of these sequences [see Measurement Method] aligned with viruses from the Anelloviridae family, including Torque Teno virus (TTV), TTV-like virus, SEN virus, TTV midi virus, and TTV-like mini virus (Table 1), which has been previously reported [5, 12, 16]. However, [researchers] unexpectedly found that 2.5% of the viral metagenome matched that of Marseillevirus. [They] were initially unconvinced that these data were correct, as this virus has only been found in [their] laboratory." |
Entered by | Uri M |
ID | 110702 |