| Range |
~10-40 channels/µm^2
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Kirichok Y, Krapivinsky G, Clapham DE. The mitochondrial calcium uniporter is a highly selective ion channel. Nature. 2004 Jan 22 427(6972):360-4. doi:10.1038/nature02246 p.363 left column 2nd paragraphPubMed ID14737170
|
| Method |
"[Researchers] patch-clamp single mitoplasts (2–5µm vesicles of inner mitochondrial membrane) isolated from
COS-7 cells (Fig. 1a, b), to measure directly whole-mitoplast and
single-channel Ca2+ currents." |
| Comments |
"...unlike many Ca2+ -selective currents, I(MiCa) [mitochondrial calcium] lacked Ca2+ -dependent inactivation at energized mitochondrial potentials (approximately -160 mV)...On the basis of an I(MiCa) density of 1,000–2,000 pA/pF, >90% open probability, and 0.5–1 pA single-channel amplitude at [Ca2+]c=105mM and Vm=-160mV, MiCa channel density in the inner mitochondrial membrane is approximately 10–40 channels per µm^2, slightly lower than voltage-gated Ca2+
channel density (about 100 per µm^2) (ref. 29) in excitable cell membranes." |
| Entered by |
Uri M |
| ID |
110626 |